Not necessarily. Preparation can proceed while funds move, provided the record shows what is already committed and what remains scheduled. Canadian citizens generally need an E-2 visa, and no processing time, appointment date, or outcome can be guaranteed. Build the timetable around evidence you control, not a bank's discretionary approvals.
Set milestones for each tranche and each commitment
Date the steps: authorization requested, funds received in the U.S. account, lease signed, equipment ordered, staff engaged. Those dates help distinguish completed transactions, binding commitments and plans still awaiting authorization. Track visa validity separately from the authorized period of stay recorded at admission, since they are distinct concepts and may have different dates. Keep the plan consistent with departing when status ends, including how the business would be handled then.
Signing the purchase agreement first fixes the price and the asset list; funding the escrow immediately afterward creates the irrevocable commitment the application needs; the visa file is then assembled around those executed documents. The standard answer is an escrow arrangement: the purchase agreement is signed, the price is deposited with an escrow agent, and release to the seller is conditioned on visa issuance, with the funds returned if the visa is refused.