Not directly. Eligibility turns on treaty nationality by citizenship, at least fifty percent treaty ownership, substantial at-risk capital in a real operating enterprise, non-marginality, and the investor's development and direction of the business. A spouse's employment matters to household planning, to intent to depart, and to any later dependent application.
Show the investor's own commitment
Explain the investor’s actual presence and control in the US enterprise; do not assume the spouse’s Canadian employment automatically changes the legal test or triggers special scrutiny. Document management responsibilities, signing authority, and time on site. Non-marginality can rest on capacity beyond a minimal living for the family or on significant economic contribution, and household income from Canada is not what satisfies that element.
Keep the two income pictures clearly separated. A useful way to keep the two pictures apart is to ask which of them would be affected if the spouse's Canadian salary stopped tomorrow. The household budget would be; the marginality analysis should not, because it examines what the enterprise itself can generate rather than what the family has to live on from other sources.
Building the answer on combined household income confuses the two and weakens the very element it was meant to support. Set out the enterprise's own projected capacity on its own figures, and keep the Canadian income where it belongs, in the explanation of how the family is supported while the two households run in parallel.