It can, if you were legally entitled to that share and the money reached you through a documented path. A joint inheritance does not become fully yours because you managed the sale. Show the allocation, the sale, and the lawful disbursement of your portion into the investment, including any intermediary account.
Entitlement is proved, not assumed
Suppose the estate left the property equally to you and your sibling, the sale closed, and both halves briefly sat in one account before being split. That single account is where questions start. Evidence of who was entitled to what, and when each half moved, answers them. If your sibling later gifts you part of their share, that gift needs its own lawful-source record, since gifted funds must still be lawfully sourced.
The fact that the enterprise can pay its bills does not prove that you contributed the required amount from a lawful source. A direct EB-5 investment does not require a regional center, but it changes what the evidence looks like. Identify actual ownership, lawful funding steps and the qualifying investment proposal. Problems arise when a legitimate asset sale is documented but the route from proceeds to the investment is incomplete.